Communities of Practice (CoP) are essential to ensure cross-learning and sharing of best practices in One Health education, research, and practice.  We envision establishment of illustrative and sustainable opportunities for One Health teaching faculty to remain engaged through mentoring support, opportunities to collaborate on specific projects such as research, development of One Health credentialing opportunities, contributions to peer-reviewed publications, and winning of competitive grants, awards, and prizes. The regional Networks will be positioned for long-term ownership and management of these virtual One Health Communities of Practice over time.